Preventing Violence Against Female Inmates Act of 2025

This bill requires federal and state prisons to house inmates based on biological sex, defined as the sex assigned at birth based on physical characteristics. It prohibits co-housing inmates of different biological sexes in the same housing unit, except for temporary, non-overnight situations. States must certify compliance to receive federal prison funding, and the federal Bureau of Prisons must implement this policy for its facilities. The bill directly affects all inmates in participating federal and state prisons by changing housing arrangements to separate by biological sex.