Protection of Women and Girls in Sports Act of 2023

This bill prohibits federally funded schools and athletic programs from allowing individuals assigned male at birth to participate in women's or girls' sports teams. It defines "sex" for this purpose solely based on biological sex assigned at birth, including reproductive anatomy and genetics. The law amends Title IX of the Education Amendments of 1972 to make such participation a violation of federal civil rights law. It directly affects all schools, colleges, and organizations receiving federal funds that operate athletic programs for women or girls.